305 Essay Example | Topics and Well Written Essays - 1000 words - 1

305 - Essay Example It will also increase efficiency because the front desk will only use automated services to communicate with the other departments. It will also ensure surveillance so that workers at the front-desk department do their responsibilities without wasting time. The front-desk department at the organization has for a long time been ineffective creating losses for the company because of a waste of time. The main issue regards doing things manually at the reception. The company secretaries do not use any machine that links the department to other departments within the company. The only machine in the front desk area is a computer that is only used to record customer visits when they come to the company and employee attendance every morning when they report to work. The front desk is supposed to receive customers and potential clients, listen to their concerns and send them to the relevant departments for help or service. However, customers and employees have reported that the front-desk is very slow in their responsibilities, ignore their duties and are ineffective. The source of this problem is that the front desk lacks automated systems that could have helped the department to make the processes fast and be reliable. Because of this, employees at the department are forced to walk to other departments in search of important documents or to seek clarification over important matters that may not be appropriate for phone calls. This has seen some employees take up to 30 minutes when going to take important documents. They waste time along the way, tell stories during work time and deliberately take time to avoid doing much work. In addition, simple tasks that require a phone call to other departments are ignored. Instead of making the calls, the employees deliberately walk out of their working area pretending that they need to go and find such information physically. These problems have increased inefficiencies within the

Public Health and Health Care Essay Example for Free

Public Health and Health Care Essay Public health is one the of the science that improves and protects the health care of the communities and families through promotions of healthy lifestyles, research for injury and disease prevention and detection and control of infectious diseases. Public health professionals try and prevent issues from happening or recurring through implementing educational programs recommending policies, manage services and conducting research. Such as contrast to clinical professionals doctors, and nurses, who will focus primarily on treating people after they become injured or ill. Public health also works to limit health care disparities. A greater part of public health is promoting health care equity, accessibility and quality (International Health Conference, 1946). How CDC Contributes to Public Health The Centers for Disease Control and Prevention (CDC) is the nation’s disease prevention and wellness promotion agency, protecting individual’s safety and health, giving credible information to enhance health care decisions, and improving health care through strong organization. Centers for Disease Control and Prevention (CDC) work includes wide range health care threats, including infectious chronic diseases, birth defects, injuries, water safety food, environmental hazards, safety and occupational health. Centers for Disease Control and Prevention (CDC) also administers funding for local health departments and state, community based companies and academic institutions for a wide array of public health programs and research. Daily Centers for Disease Control and Prevention (CDC) experts work both behind the scenes and on the frontlines to improve individual’s daily lives and respond to health care emergencies. Centers for Disease Control and Prevention (CDC) is h eadquartered in Atlanta and has just about 14,000,  full-time, part-time and contract employees located all through the U.S. and in 54 countries (Centers for Disease Control and Prevention, 2012). Specific Ways CDC Influence/ Roles of CDC Agency Centers for Disease Control and Prevention (CDC) contribute with its partner around the world to detect and investigate health care problems, monitor health, conduct research to enhance prevention, develop and advocate sound public health policies, and provide leadership and training. Centers for Disease Control and Prevention (CDC) develops a system design recognizing information and sources that best tackle a surveillance goal, be familiar with who will have rights to use information, by what technique and under what circumstances; also improving analysis or action by improving the surveillance system communication with other information systems Using data group recognizing possible bias linked with another collection technique cultural approach in the direction of technology or telephone use, recognizing suitable use of structured data compared with free text, more useful, data standards and language and suggest technologies such as global radio-frequency and positioning systems i dentification to maintain faster, higher-quality data and easier, access in the field. Information management and collation recognize ways to share data across different computing technology proposal linking new information with data from legacy systems; and be familiar with and remedying information quality issues as making certain information security and privacy. â€Å"Analysis recognize suitable statistical and image applications, put together algorithms to prepare users to aberrations within health care events also leveraging high routine computational resources for large data sets or difficult analyses† (Dixon, Gamache, Grannis, 2013). Understanding the value of evaluate information from one surveillance plan with other information time, place, person, or condition for new viewpoint and merge data of other quality to give a background for explanation and foundation. Diffusion suggest suitable let somebody see information users and the best technique to reach the proposed audience, smooth the progress of data finding; and recognize benefits for information providers (Lundstrom, Pugliese, Bartley, Cox, Guither, 2002). Application to public health programs assessing the utility of having surveillance data directly flow into information systems that support public health interventions and information elements or standards that facilitate this linkage of surveillance to action and improving access to and use of information produced by a surveillance system for workers in the field and health-care providers (Dixon, Gamache, Grannis, 2013).

Qualitative Tests Used For Carbohydrates Biology Essay

Qualitative Tests Used For Carbohydrates Biology Essay Carbohydrates are an essential component of our diet and an important source of energy for us. Most of the things generally included in our diet have a large ratio of carbohydrates present. Also the patients suffering from Diabetes excrete huge amounts of sugar in their urine which needs to be estimated. The purity of carbohydrates can also be checked by methods which can qualitatively estimate particular sugars. Hence the need for carbohydrate estimation arose. We have been estimating carbohydrates qualitatively since school time but it was just confined to observing a colour change or a coloured precipitate to detect the presence of a carbohydrate. None of us ever thought that these qualitative tests are highly sensitive and provide much more information than just detecting the presence of a sugar. In this study conducted, two qualitative tests used for carbohydrates were studied: Benedicts test and Seliwanoffs test. Benedicts test is a test used for detecting the presence of Reducing Sugars. The test was performed using Glucose which is the most common reducing sugar. Sucrose was used as a negative control. Different concentrations of glucose were tried ranging from 1.6 M to 1.6 mM and from 4% to 0.25%.Also along with concentration the amount of sugar was varied to find the sensitivity limits and the limitations of the test. The result of Benedicts test is usually a brick red precipitate but with some modifications different colours of solutions and different amounts of precipitate could be observed with slight variation in the concentration and amount of sugar. This suggests that this test being Qualitative is not only an indicator of the presence or absence of a reducing sugar but can also be used to roughly estimate the concentration of sugar present. It can be very useful in estimation of the concentration of sugar present in the urine of diabetic patients. Seliwanoffs test is a qualitative test used for distinguishing between Aldoses and ketoses. Ketoses form a cherry red condensation product whereas Aldoses react to form a blue-green condensation product, which may further change to a peach product. The test was performed using fructose as the sample sugar. Various concentrations of Fructose were used ranging from 4% to 0.01% to find the sensitivity limits and the limitations of the test. The test appeared to be sensitive even at 0.01% showing a very faint red colour. There was a huge variation in the intensity of colour obtained at different concentrations of sugar. But the drawback of the test was that the red colour of the solution was not stable. It intensified with increase in the duration of time. 2. INTRODUCTION Carbohydrates are the most abundant bio molecules on Earth. Each year, photosynthesis converts more than 100 billion metric tons of CO2 and H2O into cellulose and other plant products. Certain carbohydrates (sugar and starch) are a dietary staple in most parts of the world, and the oxidation of carbohydrates is the central energy-yielding pathway in most non-photosynthetic cells. Insoluble carbohydrate polymers serve as structural and protective elements in the cell walls of bacteria and plants and in the connective tissues of animals. Other carbohydrate polymers lubricate skeletal joints and participate in recognition and adhesion between cells. More complex carbohydrate polymers covalently attached to proteins or lipids act as signals that determine the intracellular location or metabolic fate of these hybrid molecules, called glycoconjugates. .Carbohydrates are polyhydroxy aldehydes or ketones, or substances that yield such compounds on hydrolysis. Many, but not all, carbohydrates have the empirical formula (CH2O)n; some also contain nitrogen, phosphorus, or sulphur. There are three major size classes of carbohydrates: monosaccharides, oligosaccharides, and polysaccharides Classification Carbohydrates can be classified on the basis of the various characteristics they possess. They can be classified on the basis of the number of carbon atoms in the sugar chain, the terminal functional group in the chain, the number of sugar subunits and the reducing activity of the sugar units. Depending on various basis they are of several types: A. According to the number of carbon atoms in the sugar chain: 1. Trioses: contain 3 carbon atoms (e.g. glyceraldehyde). 2. Pentoses: contain 5 carbon atoms (e.g. ribose). 3. Hexoses: contain 6 carbon atoms (e.g. glucose). B. According to the terminal functional group in the sugar chain: 1. Aldoses: contain terminal aldehyde group (-CHO) (e.g. glucose). 2. Ketoses: contain terminal ketone group (C=O) (e.g. fructose). C. According to the number of sugar subunits: 1. Monosaccharides: Monosaccharides, or simple sugars, consist of a single polyhydroxy aldehyde or ketone unit. The most abundant monosaccharide in nature is the six-carbon sugar D-glucose, sometimes referred to as dextrose. 2. Oligosaccharides: Consist of short chains of monosaccharide units(2-10) joined by characteristic linkages called glycosidic bonds. The most abundant are disaccharides, with two monosaccharide units. e.g. sucrose (cane sugar), which consists of the six-carbon sugars- D-glucose and D fructose. 3. Polysaccharides: The polysaccharides are sugar polymers containing more than 20 or so monosaccharide units, and some have hundreds or thousands of units. Some polysaccharides, such as cellulose, are linear chains; others such as glycogen, are branched. Both glycogen and cellulose consist of recurring units of D-glucose, but they differ in the type of glycosidic linkage and have different properties and biological roles. . D. According to the reducing activity of the sugar unit: Carbohydrates that can undergo oxidation are called reducing sugars. This depends on the presence of an exposed carbonyl group. 1. Reducing sugars: Certain sugars with a free carbonyl carbon can be oxidized by oxidizing agents such as ferric (Fe3) or cupric (Cu2) ion. The carbonyl carbon (anomeric carbon) is oxidized to a carboxyl group. Such sugars capable of reducing ferric or cupric ion are called Reducing sugars. e.g. lactose, maltose. 2. Non-reducing sugars: Sugars like sucrose contain no free anomeric carbon atom since the anomeric carbons of both monosaccharide units are involved in the formation of glycosidic bond. Therefore it is a Non-Reducing sugar. 2.1 Biochemical Tests for Carbohydrates The biochemical tests for carbohydrates can be divided into two categories: Qualitative Tests- These tests are performed to detect the presence of carbohydrates in a test sample. They are also used to detect the type of carbohydrate present. There are different types of qualitative tests for different types of carbohydrates. e.g. Fehlings and Benedicts test for reducing sugars, Seliwanoffs test for ketoses, Iodines test for starch,Bials test for detecting pentoses etc. They are not very sensitive as compared to quantitative tests and cannot estimate the exact amount of carbohydrates present but show some difference in the observation with variation in the amount of carbohydrate hence, can be used to make a rough estimate of the amount of sugar present. Quantitative Tests- These tests are the advanced form of qualitative tests and can be used to estimate the amount of carbohydrate present in a given sample. These tests use particular chemicals which form coloured complexes with sugars which can then be read at certain wavelengths using a spectrophotometer. Their absorbance can then be used to estimate the exact amount of carbohydrate present in the sample. e.g. Anthrone test, DNS Method, Phenol- Sulphuric Acid Method etc. 2.11 Qualitative Test Flow Chart for classifying an unknown carbohydrate A. Molisch Test Molisch test is used to distinguish between carbohydrates and non- carbohydrates. It is the preliminary test used to detect the presence of carbohydrates in a sample. Principle It uses concentrated sulphuric acid as a Dehydrating acid which dehydrates all carbohydrates to form Furfural or 5-hydroxymethylurfural from reaction of sulphuric acid with pentoses and/or hexoses. These products condense with ÃŽ ±-naphthol to yield a purple condensation product. B. Iodine and Potassium Iodide Test This is a test used particularly to detect starch and glycogen. Starch gives a blue-black colur with potassium iodide whereas glycogen gives reddish-brown colour. Principle Starch contains ÃŽ ±-amylose, a helical saccharide polymer, and amylopectin. Iodine forms a large complex polysaccharide with the ÃŽ ±-amylose helix, producing a blue-black colour. Simple Oligosaccharides and Monosaccharides do not form this complex with Iodine. Thus, the I2/KI test can be used to distinguish starches from other carbohydrates. C. Bials Test Bials test is used to distinguish between pentoses and hexoses. Principle This test uses concentrated hydrochloric acid as the dehydrating acid and orcinol with a trace of iron(III) chloride as the condensation reagent.. Pentoses subjected to the test yield a blue or green condensation product, while hexoses yield a muddy brown to grey condensation product. Pentose Dehydration Product Blue or Green condensation product (Furfural) Hexose Dehydration Product Muddy brown-Grey condensation product (5-hydroxymethylfurfural) D. Seliwanoffs Test Seliwanoffs test is used to distinguish between aldoses and ketoses. Principle This test uses 3N hydrochloric acid as the dehydrating agent as resorcinol as the condensation reagent. When mixed with Seliwanoffs Reagent, Ketopentoses react within 2 minutes to form a cherry red condensation product. Aldopentoses react after 2 minutes to form a blue-green condensation product, which may further change to a peach product. Fructose Hydroxy-methyl Cherry-Red Complex Furfural Identifying Reducing Sugars All mono and disaccharides with a free aldehyde or keto group act as reducing agents in alkaline solutions. The reducing properties of sugars are dependent upon the presence of actual or potential aldehyde or ketone groups. The enolization of sugars under alkaline conditions is an important consideration in reduction tests. The ability of a sugar to reduce alkaline test reagents depends on the availability of an aldehyde or keto group for reduction reactions. A number of sugars, especially disaccharides or polysaccharides have glycosidic linkages which involve bonding between each group, and hence there is no reducing group on the sugar; such as the case for sucrose, trehalose, inulin, glycogen, starch, and dextrin. In the case of reducing sugars, the presence of alkali causes extensive enolization especially at high pH and temperature. This leads to a higher susceptibility to oxidation reactions than at neutral or acidic pH. These sugars, therefore, become potential agents capable of reducing Cu+2 to Cu+, Ag+ to Ag and so forth. Reducing sugars can react with many different oxidizing agents. Fehlings test, Benedicts test and Barfoeds test have been used to distinguish between monosaccharides and disacc harides Monosaccharides fluctuate between a ring open form and a ring closed form. The ketone (-C=O) group, for Fructose and the aldehyde group (-CHO), for Glucose in the ring open forms can be reduced using these tests. Some sugar units in disaccharides also fluctuate between a ring open form and a ring closed form. These disaccharides are also reducing sugars because the ring open form has a ketone or aldehyde to react. Sucrose is one of the few disaccharides that do not have a ring open form so it is a non-reducing sugar.  ­Ã‚ ­Ã‚ ­ Ring Open Fructose and Ring Closed Fructose Ring Open Glucose and Ring Closed Glucose Reducing Sugars are oxidized by copper (II) ions. Benedicts reagent and Fehlings Reagent are mildly basic solutions whereas Barfoeds Reagent, a mildly acidic solution. The presence of red copper (I) oxide precipitate indicates that the saccharide has reduced the copper (I) ions. E. Fehlings test Principle Fehlings test uses a mixture of fehlings solution A and B. Fehlings solution A consists of copper(II) sulphate dissolved in dilute sulphuric acid. Fehlings solution B is sodium potassium tartarate dissolved in dilute NaOH. Both the solutions are mixed in equal proportions and used as fehlings reagent. This reagent is used as a general test for detecting reducing sugars. A reducing sugar reduce copper(II) ions to copper(I) oxide, forming a red precipitate. CuSO4Cu++ + SO4 2 Cu++  + Cu+ Glucose (Reducing Sugar) Cu+Cu2O (red precipitate) Cuprous Oxide F. Benedicts test Principle Benedicts test uses a mixture of copper(II) sulphate, sodium citrate, and sodium carbonate in a mildly basic solution. This reagent is used as a general test for detecting reducing sugars. A reducing sugar reduce copper(II) ions to copper(I) oxide, forming a red precipitate. CuSO4Cu++ + SO4- (Copper Sulphate) (Cupric Ion) (Sulphate Ion) 2 Cu++  + Cu+ Glucose (Reducing Sugar) Cu+Cu2O (red precipitate) Cuprous Oxide G. Barfoeds Test Benedicts test gives positive test results for all reducing sugars. However, not all reducing sugars react at the same rate. With different oxidizing agents, disaccharides are considerably less reactive compared to monosaccharides. A positive Barfoeds test result is similar to that observed with Benedicts solutions. Monosaccharides give positive Barfoeds test results within 2-3 minutes, while disaccharides do not react under the same conditions. Principle Barfoeds test uses copper(II) ions in a slightly acidic medium. If the reaction time is carefully monitored, this test can be used to distinguish reducing monosaccharides from reducing disaccharides. Reducing disaccharides cause the formation of copper(I) oxide after approximately 10 minutes. (CH3COO) 2Cu + 2H2O 2 CH3COOH + Cu(OH)2 Cu(OH)2 CuO+ H2O 2CuO  +Cu2O (red precipitate)   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   (Glucose) Reducing Sugar 2.12 Quantitative Tests A. Determination of Total Carbohydrate by Anthrone Method Principle Carbohydrates are first hydrolysed into simple sugars using dilute hydrochloric acid. In hot acidic medium glucose is dehydrated to hydroxymethyl furfural. This compound forms with anthrone a green coloured product with an absorption maximum at 630 nm. HCl anthrone Carbohydrate furfural/hydroxymethyfurfural Green product (630 nm) B. Phenol Sulphuric Acid Method for Total Carbohydrate Principle In hot acidic medium carbohydrates are dehydrated to furfural/hydroxymethyl furfural. This forms a green coloured product with phenol and has absorption maximum at 490 nm. H2SO4 phenol Carbohydrate furfural/hydroxymethylfurfural Green product (490 nm) C. Determination of Reducing Sugars by Nelson-Somogyi Method The Nelson-Somogyi method is one of the classical and widely used methods for the quantitative determination of reducing sugars. Principle The reducing sugars when heated with alkaline copper tartarate reduce the copper from the cupric to cuprous state and thus cuprous oxide is formed. When cuprous oxide is treated with Arsenomolybdic acid, the reduction of molybdic acid to molybdenum blue takes place. The blue colour developed is compared with a set of standards in a colorimeter at 620 nm. Cu2O+ Arsenomolybdate Molybdenum Blue (Cuprous Oxide) (500 nm) D. Estimation of Reducing Sugar by Dinitrosalicylic Acid Method This method is an alternative to Nelson-Somogyi method. It is a simple, sensitive and adoptable method during handling of a large number of samples at a time. However,  enzymatic  methods are usually preferred to DNS due to their specificity. Principle 3,5-Dinitrosalicylic acid   is an  aromatic compound  that reacts with reducing sugars to form  3-amino-5-nitrosalicylic acid, which absorbs  light  strongly at 540  nm. 3,5-Dinitrosalicylic acid 3-amino-5-nitrosalicylic acid E. Determination of Glucose by Glucose Oxidase Method Glucose is a widely distributed simple sugar with an active aldehyde group. Estimation of glucose by glucose oxidase gives the true glucose concentration eliminating the interference by other reducing sugars. Principle Glucose oxidase catalyses the oxidation of alpha-D-glucose to D-glucono-1, 5 lactone (gluconic acid) with the formation of hydrogen peroxide. The oxygen liberated from hydrogen peroxide by peroxidase reacts with the O-dianisidine and oxidises it to a red chromophore product. Glucose + O2 H2O2 + Gluconic Acid (glucose oxidase) H 2O2 + O-dianisidine Red-coloured product (peroxidase) (540 nm) 3. MATERIALS REQUIRED I) Stock Solutions Glucose 1.6 M Glucose 4% (w/v) Sucrose 0.1 M Fructose 4% (w/v) II) Reagents Benedicts Reagent Step 1-Dissolve 173 g sodium citrate and 100 g sodium carbonate in about 500 mL water. Step 2-Heat to dissolve the salt Step 3-Dissolve 17.3 g copper sulphate in about 100 mL water and add it to the above solution with stirring and make up the volume to 1 L with water. Seliwanoffs Reagent Dissolve 0.05 gm resorcinol in 3N hydrochloric Acid. III) Miscellaneous Water Bath Test Tubes Clamps Conical Flasks Beakers Containers Test tube holders 4. METHODOLOGY 4.1 Benedicts Test Step 1- Pipette out 1 ml of glucose solution in some test tubes so that there is a triplicate for each set. Step 2- Pipette out 1ml of Distilled Water in one test tube which serves as blank and 1 ml of Sucrose solution (0.1M) in another which serves as a negative control. Step 3- Add 2 ml of Benedicts reagent in all the test tubes. Step 4-Transfer all five tubes to the boiling water bath provided and record the time for the appearance of precipitate. Step5- Make a subjective analysis of the colours observed. A scale of + to +++++ can be used to record the depth of the colour, from lightest to darkest. 4.11 Effect of variation in Carbohydrate Concentration (in M) The concentration of glucose was varied from 1.6 M to 1.6 mM keeping the reaction volume constant to 3 ml. The upper and the lower sensitivity limits and the optimum molar concentration of glucose was found for the test through subjective analysis. 4.12 Effect of Reaction Volume The reaction volume was reduced from 3 ml to 1.5 ml for all the concentrations of glucose ranging from 1.6 M to 1.6 mM to find any difference in the sensitivity range and the optimum molar concentration of glucose for the test. 4.13 Effect of variation in Carbohydrate Concentration (in %) The concentration of glucose was varied from 4% to 0.25%. Also the reaction volume was changed and the analysis was performed in two sets. The volume of reagent used was 5 ml whereas the volume of samples all concentrations in Set A and B were 50ÃŽ ¼l and 25ÃŽ ¼l respectively. 4.14 Effect of variation in Sample Volume The volume of glucose was varied for the same concentrations 4% to 0.25% to see the changes in the colour of solution obtained and at the same time find the upper and lower limits at which the test still remains sensitive. The analysis was performed in 6 sets with the following volumes of glucose: 200ÃŽ ¼l, 100ÃŽ ¼l, 50ÃŽ ¼l, 25ÃŽ ¼l, 12.5ÃŽ ¼l and 6.25ÃŽ ¼l. 4.15 Effect of Reaction Volume on the results obtained. Of the various sets tried 5 ml of Benedicts reagent and 200ÃŽ ¼l of sample gave the best results. To verify the consistency of results obtained the reaction volume was reduced. The analysis was performed in two sets. Set A with 2.5 ml of reagent and 100ÃŽ ¼l of sample and Set B with 1.25 ml of reagent and 50ÃŽ ¼l of sugar sample. 4.2 Seliwanoffs Test Step 1- Pipette out 1 ml of fructose solution in test tubes so that there is a triplicate for each set. Step 2-Pipette out 1ml of Distilled Water in one test tube which serves as blank. Step 3- Add 2 ml of Seliwanoffs reagent in all the test tubes. Step 4-Transfer all tubes to the boiling water bath for 2.5 mins. Step 5- Make a subjective analysis of the colours observed. A scale of + to +++++ can be used to record the depth of the colour, from lightest to darkest. 4.21 Effect of variation in carbohydrate concentration (in %) The concentration of fructose was varied from 4% to 0.015% keeping the reaction volume constant to 3 ml. The upper and the lower sensitivity limits and the optimum concentration of fructose were found for the test through subjective analysis. 5. OBSERVATIONS 5.1 Benedicts Test 5.11 Effect of variation in Carbohydrate Concentration (in M) A brick red precipitate was observed for all the concentrations of sample ranging from 1.6 M to 3.125 mM. But the last concentration of 1.6 mM showed a negligible amount of precipitate. Also once centrifuged the supernatant was found to be colourless for concentrations ranging from 1.6 M to 0.1 M. The supernatant was observed to be blue in concentrations ranging from 50 mm to 1.6 mM. Glucose (M) Colour Intensity 1.6 0.8 0.4 0.2 0.1 0.05 0.02 0.012 0.006 0.003 0.001 +++ ++++ ++++ +++++ ++++++ +++++ ++++ +++ ++ + 5.12 Effect of variation in Reaction Volume A brick red prcipitate was observed for all the concentrations of sample ranging from 1.6 M to 6.25 mM. Concentrations 3.125 mM and 1.5625 mM showed a negligible amount of precipitate. For concentrations 6.25 mM to 0.1 M there was an increase in the amount of precipitate observed with the highest amount formed in 0.1 M of sample. For the next two concentrations i.e. 0.2 M and 0.4 M the amount of precipitate formed was almost equal but again reduced for 0.8 M and 1.6 M glucose sample. Also after centrifugation the supernatant was found to be colourless for concentrations ranging from 1.6 M to 0.1 M. The supernatant was observed to be increasingly blue in concentrations ranging from 50 mm to 1.5625 mM. Glucose (M) Colour Intensity 1.6 0.8 0.4 0.2 0.1 0.05 0.02 0.012 0.006 0.003 0.001 +++ ++++ +++++ +++++ ++++++ ++++ +++ ++ + 5.13 Effect of variation in carbohydrate concentrations (in %) There was a brick red precipitate observed in for all the concentrations of sample ranging from 0.25% to 4% in both the sets. The highest amount of precipitate was observed for 1% sample but there was no consistency in results seen. Glucose (%) Set Set B 4.0 2.0 1.5 1.0 0.5 0.25 +++ + ++++ + ++ + +++ 5.14 Effect of variation in Sample Volume There was a variation in the colours obtained at different concentrations and volumes of sugar sample used. But sets with 12.5ÃŽ ¼l and 6.25ÃŽ ¼l of sugar did not show any noticeable changes in colour. Glucose (%) Set A Set B Set C Set D Set E Set F Set B Set C Set D Set E Set F 4.00 2.00 1.00 0.50 0.25 reddish brown brown green greenish blue dull blue brown green greenish blue dull blue green greenish blue dull blue blue blue greenish blue dull blue blue blue blue dull blue blue blue blue blue blue blue blue blue blue 5.15 Effect of reduction in the reaction volume There was no change in the observations due to reduction in the reaction volume. Glucose (%) Set A Set B 4.0 2.0 1.0 0.5 0.25 reddish brown brown green greenish blue dull blue reddish brown brown green greenish blue dull blue 5.2 Seliwanoffs Test 5.21 Effect of variation in carbohydrate concentration (in %) A cherry red colour is observed for all the concentrations of fructose used but the intensity of colour obtained decreases with decrease in concentration. The lowest concentration used (0.015%) has a faint red colour. Also the colour of the solution intensifies with time if kept after boiling. Fructose (%) Colour Intensity 4.00 2.00 1.00 0.50 0.25 0.12 0.06 0.03 0.01 +++++++++ ++++++++ +++++++ ++++++ +++++ ++++ +++ ++ + 6. RESULTS AND DISCUSSION 6.1 Benedicts Test 6.11 Effect of variation in Carbohydrate Concentration (in M) It can be observed that 0.1 M is the optimum concentration of sugar for Benedicts reaction with the reaction volume of 3 ml since the highest amount of precipitate is formed at 0.1 M. Also the supernatant obtained after centrifugation is colourless which suggests that the reaction is completed and there is no unused reagent left. Concentrations less than 0.1 M show decreasing amounts of precipitate and the colour of the supernatant is also increasingly blue. This suggests that as the concentration of sugar is lowered the amount of unreacted Benedicts reagent increases which leaves the solution blue even after the reaction completes. 1.6 mM sugar sample shows a negligible amount of precipitate formation which suggests that the reaction is not sensitive for concentrations lower than 1.6 mM. For concentrations higher than 0.1 M the amount of precipitate formed again decreases with increase in concentration which suggests that the concentration is too high as compared to the amount of reagent used and hence no more precipitate is formed after the reaction completes. 6.12 Effect of variation in Reaction Volume The reaction volume was reduced to half but has no effect on the results of the experiment. 0.1 M is the optimum concentration of sugar for the reaction with the reaction volume of 3 ml since the highest amount of precipitate is formed at 0.1 M. Also the supernatant obtained after centrifugation is colourless which suggests that the reaction is complete and there is no unused reagent left. Concentrations less than 0.1 M show decreasing amounts of precipitate and the colour of the supernatant is also increasingly blue. This suggests that as the concentration of sugar is lowered, the amount of unreacted reagent increases which leaves the solution blue even after the reaction completes. 3mM and 1.6 mM sugar samples show a negligible amount of precipitate formation which suggests that the reaction is not sensitive for concentrations lower than 3 mM with a reaction volume of 1.5 ml. For concentrations higher than 0.1 M, the amount of precipitate formed again decreases with increase in concentration which suggests that the concentration is too high as compared to the amount of reagent used and hence no more precipitate is formed after the reaction completes. 6.13 Effect of variation in carbohydrate concentrations (in %) The highest amount of precipitate was formed in 1% sugar sample but there was no consistency in the observations due to incorrect preparation of reagent. During the preparation of reagent instead of mixing Sodium citrate and Copper Sulphate together in hot water they were dissolved separately and then mixed. Also the solution was not made up to 500 ml with distilled water rather measured amount of water was added to the solution to make it 500 ml which made the solution dilute and hence gave incorrect results. 6.14 Effect of variation in Sample Volume Different volumes of sugar were tried for all the different concentrations. There was a variation in the colours obtained. 200ÃŽ ¼l of sugar reacted with 5 ml of reagent showed maximum variation in colours at different concentrations: Reddish Brown, Brown, Green, Greenish blue and dull blue obtained at 4%, 2%, 1%, 0.5% and 0.25% respectively. Sets with 12.5ÃŽ ¼l and 6.25ÃŽ ¼l of sugar volume did not show any noticeable changes in colour which suggest that the volume of sugar solution was not enough for the reaction to take place. 6.15 Effect of reduction in the reaction volume Reduction in the reaction volume made no difference to the variation in colours obtained at different concentrations of sugar which suggests that Benedicts reagent and glucose can be reacted in this ratio in any volume to estimate the concentration of glucose present in an unknown sample. 6.2 Seliwanoffs Test 6.21 Effect of variation in carbohydrate concentration (in %) A cherry red colour is observed for all the concentrations of Fructose used but the intensity of colour obtained decreases with decrease in concentration. The highest intensity is observed at 4% sugar concentration. 0.015% which is the lowest concentration of sugar used gives a very faint red colour which cannot be taken as pos

Why Drugs Should Be Legal :: essays research papers

  Ã‚  Ã‚  Ã‚  Ã‚  America is wasting it’s money and resources. It’s trying restrict something on which restrictions don’t have any effects: drugs. People who don’t use recreational drugs don’t do so because of the health risks; people who do use drugs would whether or not they are legal. The fact that they are illegal makes little difference. Nevertheless, $15,000,000,000 goes directly into drug prohibition every year, and has very little effect. Very much money is spent to pay police narcotics officers, fund the D.E.A., and house drug-offenders in prison. The prisons are full of drug-related criminals, and violent offenders go free earlier because of this. Which would you prefer walking the streets, a rapist, or a potsmoker?   Ã‚  Ã‚  Ã‚  Ã‚  Legalizing recreational drugs does not mean making drugs accesible to all people. The drugs that are legal today, alcohol and tobacco (nicotene) aren’t available to just everyone; they are regulated. Only certain people are allowed to buy them. Since the drug trade is unregulated, drugs are sold anywhere they can be (e.g. schools), allowing children to have access to them. If these drugs were illegal, than that trade would stagnate, and children wouldn’t have such easy access to them. It makes sense†¦ do you ever see people in schools selling beer or cigarrettes? Also, the usual cause of drug overdose is the fact that a person cannot know the potency of the drug he/she is taking. There are no standards because the trade of drugs is illegal in the first place. If they were legal, there would be a standard of quality for all drugs, regulated by the FDA.   Ã‚  Ã‚  Ã‚  Ã‚  The state of Georgia has the highest excise tax on liquor of any state. It also has the lowest tax on gasoline (which is good especially now with gas prices the highest ever). If drugs were legal, the government could place such an excise tax on them. In addition to freeing up money used for their prohibition, this extra money could fund the real problems of America, and eliminate our national debt quite quickly.   Ã‚  Ã‚  Ã‚  Ã‚  Doing drugs is dangerous, yes†¦ but so is tanning, smoking, drinking, chewing tobacco†¦ the list goes on and on. People are still free to do these things, notwithstanding the fact that they may be stupid acts. The ratio of deaths from tobacco versus drugs is 425:1, and that of alcohol versus drugs is 50:1. Drugs are less dangerous than both of these legal things, and people are still free to do them and not free to do drugs?

My Childhood Hero Essay

Every child has a hero, someone they look up to, someone who guides their thoughts and influences their life. My childhood hero was Belle from the fairytale, The Beauty and the Beast. As a child, I watched the movie end to end and read the book countless times, losing myself in her magical world so completely that I actually began to know the lines by heart. I never once got bored of it and frankly, right until today, Belle is still my inspiration. Though I first admired her for her outer beauty, I then began to look up to her for her inner beauty. She was portrayed as such a happy young woman, helpful and loving and with a warm smile for all. Her ability to be everyoneÂ’s friend, to sing such delightful songs always made me happy and feel like part of her world, not just an on-looker. I actually used to wish that Belle would come alive and be my friend. However, the main reason Belle became my hero was because of her sacrifice. Her willingness to let go of all she knew and loved to save her father has never ceased to impress me. This truly showed me the depth of her love for her father and taught me the value of family and parents. Besides depicting BelleÂ’s loving side, her sacrifice also gave me an insight to her courage. She braved the hound dogs in the mountains and continued with what she started out for, as a sacrifice to the beast who resided in a huge, deserted palace in the loneliest part of the mountains. It was through Belle that I learnt the importance of patience, endurance and perseverance. She had the courage to face the beast, to endure his fearful sight and to bear the brunt of his volatile temper just to save her fatherÂ’s life. Not only that, Belle was also able to see through physical countenances and fall in love with the ugly beast instead of shunning him away and marrying the handsome but shallow Gaston. To me, Belle was and still is someone I want to be. I always wished to grow up to be the way Belle was. I related to her through our common passion for books and loved her for who she was. Through BelleÂ’s final victory where the beast transformed back to a Prince and Gaston got defeated, she became my image of how the good and righteous always triumphs over the evil. The magic  within the tale and the “happily ever after” ending has given me a reason to belief in the unbelievable, to have faith and truly trust the existence of a greater power. The true love between the Beauty and the Beast is why I dream of my Prince charming finding me one day. BelleÂ’s heart of gold made her my childhood hero. Ten years have passed and unabashedly I confess that she is still my role model and I still do hope that I am able to be as admirable as her. Who says fairy tales are for kids?

Dear Doug 1

Dear Doug I am glad to hear that you are settled in and that everything is well. It is also a relief to hear that you and your roommate seem to be getting along and I hope that your friendship with Nathan will grow. I have thought about your questions and decided on some ways to help you explain, and help Nathan interpret and understand Christianity. In my life I have experienced the same situation you mentioned about Christian terminology and what it means to be a Christian.I grew up in a Christian home under Christian principles and it had not occurred to me â€Å"why do I believe in Christianity†? Of course this did not occur to me until I was older and had thought about it for some time. I thought about this world and how it could have been created not by mere chance but by something or someone far more intelligent than mere humans, not by chance. This universe and everything in it was created with superior intelligence and for a purpose.I looked into Christianity and saw how God created the earth; a being far above humans because we were made by him; a perfect powerful all knowing being who created us in his likeness to serve a distinct purpose in this world, rather than leaving it up to mere chance. I knew there had to be a reason for this world and a good one! I looked farther into Christianity and the story of how God created the earth called the Gospel. I am sure you have heard of the Gospel and read the story, but you must take into account many things before you explain it to a non-Christian.Many non-Christians are not familiar with Christian terminology because they have not looked into Christianity and read the Bible to understand our belief, and we as Christ’s followers must explain the Gospel in a simplified way for people who are not familiar with certain Christian terminology so they can understand the concept and become more familiar with the terminology. One way you can simplify the meaning of these words is to define them and s implify them in a way that you clearly present the meaning of that word.An example would be instead of saying we are sinful people, you could say we are disobedient or you could define sin as bad behavior or actions that you commit. Use words that people use on regular bases to define a certain word, which will give them a better understanding since they already understand the correct meaning of that word to better understand any Christian terminology. You may not have known, but you have already or at least started to formulate and create your own worldview. A worldview is exactly what it sounds like, it’s the way we interpret, understand, and our opinion about the world and how we view it.A worldview is how you see the world and your opinion or idea of reality. Norman Geisler and William Watkins give a very well thought out worldview explanation and an example that may help you understand, â€Å"It is an interpretive framework through which or by which one makes sense of t he data of life and the world. † A worldview is like a pair of glasses and the only way to view the world and everything in it, is through the pair of glasses, and like the prescription of glasses, everyone’s worldview is different. Now that I have given you an understanding of a worldview I will do my best to explain to you what a Christian worldview is.A Christian worldview would basically be a Christian influence on how you shape your worldview. A Christian worldview would be based on Christian principles that you’ve read and learned about in your life. How you view the world and reality would be based on Christian morals and ethics rather than a different world view based on society’s standards. A Christian worldview is typically a brighter more positive outlook on life rather than a worldview not based on Christianity; it gives you more of a purpose and reason for the questions what? , why? , and how? Your last question I also struggled with and still do from time to time.We all know that we have accepted Christ into our hearts but the greater reason is why. I have asked this question to myself many times and sometimes feel over whelmed, but there is a reason. Take a look around this world; it is full of proof and purpose. The intelligence that was required to form this world was more than mere chance but above normal human intelligence. God a supernatural being who is perfect created this earth for a purpose and with intelligent design. You yourself were made for a purpose and you yourself are far more complex to be created out of mere chance.Also the sacrifice that God made for us to have another chance even though we wronged him, beyond human means of forgiveness, it amazes me how someone could love us so much; still wanting to obtain a relationship with us. Even when we show disobedience and repetitively wrong God he will still forgive us if we truly and honestly ask for forgiveness from him. He gave us free will to make our own decisions to disobey him or to love him. I hope that this letter has assisted you in understanding your questions and I would be glad to attempt and answer more, so keep thinking and writing! I hope everything with your roommate goes well, Daniel